In the first of three day-night internationals in Lahore, Pakistan captain Babar Azam won the toss and put Australia to bat.
Pakistan’s pace spearhead Shaheen Shah Afridi is out after injuring his knee while batting on Monday.
Mohammad Wasim Junior, a fast bowler, and Zahid Mahmood, a leg-spinner, are making their ODI debuts.
Already-depleted Australia lost Ashton Agar when the spinner tested positive for Covid-19 on Tuesday, a day after reserve wicketkeeper Josh Inglis was infected with the virus.
All-rounder Mitchell Marsh is also missing, having injured his hip.
The next two ODIs will be on Thursday and Saturday, also in Lahore.
Teams
Pakistan: Babar Azam (captain), Fakhar Zaman, Haris Rauf, Hasan Ali, Iftikhar Ahmed, Imam-Ul-Haq, Khushdil Shah, Zahid Mahmood, Mohammad Rizwan, Mohammad Wasim Junior, Saud Shakeel
Australia: Aaron Finch (captain), Sean Abbott, Alex Carey, Nathan Ellis, Cameron Green, Travis Head, Marnus Labuschagne, Ben McDermott, Marcus Stoinis, Mitchell Swepson, Adam Zampa
Umpires: Aleem Dar (PAK) and Asif Yaqoob (PAK)
Tv umpire: Ahsan Raza (PAK)
Match referee: Muhammad Javed Malik (PAK)
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